Demonstration of circularization of herpes simplex virus DNA following infection using pulsed field gel electrophoresis.

In this reaction, the exonuclease resects DNA from an exposed double-stranded end, and the SSB anneals complementary single-stranded regions. We have used homologous Red/ET recombination in Escherichia coli to introduce wild-type and specifically mutated Uc-DR1-Ub fragments into an ectopic site of a cloned HSV-1 genome from which the resident packaging signals had been previously deleted. This DNA lacked terminal restriction fragments and could be converted to unit-length linear species by gamma-irradiation, demonstrating the circularization of viral DNA following infection. HSV-1 alkaline nuclease, encoded by the UL12 gene, is a 5′–>3′ exonuclease that shares homology with Redalpha, commonly known as lambda exonuclease, an exonuclease required for homologous recombination by bacteriophage lambda. The genome possesses around 75 genes, generally densely arranged and without long range ordering. Cells exhibited reduced type I IFN production in response to cytosolic DNA in the absence of cGAS. Animal herpes viruses all share some common properties.

Herpes simplex virus 1 (HSV-1), a typical human-restricted DNA virus, is capable of counteracting host immune responses via several distinct viral proteins, thus establishing a lifelong latent infection. The envelope is joined to the capsid by means of a tegument. If you continue without changing your settings we’ll assume you’re happy. Poxvirus encodes its own machinery for genome transcription, a DNA dependent RNA polymerase, which makes replication in the cytoplasm possible. The matrix or tegument which contacts both the envelope and the capsid contains at least 15-20 proteins. Mikrobiologien beskæftiger sig med bakterier, virus og andre mikroorganismer. Herpes viruses cause a wide range of latent, recurring infections including oral and genital herpes, cytomegalovirus, and chicken pox.


These ‘pseudotypes’ with the envelope antigens of one virus and the genome of the other have been demonstrated for simian virus 5 and vesicular stomatitis virus (VSV)1, for avian or murine RNA tumour viruses and VSV2–4, and for fowl plague virus and VSV5. Replication initiator proteins in other systems, including those of other DNA viruses, are known to be regulated by phosphorylation; however, the role of phosphorylation in OBP function has been difficult to assess due to the low level of OBP expression in HSV-infected cells. Normally, the replication proteins accumulate within replication compartments, which expand as viral DNA synthesis increases. Some of the most well-known viruses, such as those that cause herpes, smallpox, hepatitis and warts, have a DNA based genome. The SGVs were developed to generate vaccines capable of expressing multiple genes from a single construct, which could be of great benefit for commercialization. A deletion analysis of this region showed that IRES activity was due to stem-loops VII and VIII. Aloni and Attardi showed that similar symmetrical transcription occurs in HeLa cell mitochondria4,5.

With the onset of viral genome amplification, these proteins become redistributed into large globular replication compartments. In this study, we demonstrate that the VP24 protein, a serine protease of HSV-1 essential for the formation and maturation of capsids, is a novel antagonist of the beta interferon (IFN-β) pathway. Several processes contribute towards maintaining this level of fidelity including the nucleotide selectivity of the replicative DNA polymerase, proofreading exonuclease activity, post- replication repair to correct mis-incorporation events, and other DNA repair processes such as base excision repair. The morphology of virus DNA molecules at different stages of the virus growth cycle in BSC 1 cells, was studied by electron microscopy after separation of virus DNA from cellular DNA by centrifugation in CsCl gradients. Although cGAS/STING-mediated DNA-sensing signal is crucial for host defense against many viruses, especial for DNA viruses, few viral components have been identified to specifically target this signaling pathway. The architecture of the HSV-1 virion consists of a trilaminar lipid envelope, an amorphous layer known as the tegument, a capsid shell, and a DNA-containing core. D.

Common precursor pathways of Herpes DNA and of repair synthesis in ultraviolet irradiated cells. Furthermore, viral proteins, such as viral protein 35 (VP35) from EBOV, non-structural protein 1 (NS1) or PB2 from influenza A virus (IAV) and the E3 protein from vaccinia virus, or host proteins (such as La) bind to viral RNA to inhibit the recognition of pathogen-associated molecular patterns (PAMPs) by RIG-I. The immune system protects against viral infections through coordinated innate and adaptive immune responses. We found evidence that IFI16 plays a direct role in HSV DNA sensing, whereas cGAS produces low amounts of cGAMP and promotes the stability of IFI16. Methods: Eight-week-old male BALB/c mice were inoculated via the eye by 104 plaque forming unit of wild type Iranian isolates of HSV-1. On the other hand, we have also shown that ATR and its scaffolding protein, ATRIP, are recruited to viral replication compartments, where they play beneficial roles during HSV-1 replication. However, after entry into the infected cell nucleus, the HSV genome begins to associate with nucleosomes during the earliest stages of infection.

Its widespread prevalence amongst human population, its ability to spread an asymptomatic epidemic and the lack of an effective vaccine makes the virus clinically significant.