Sequence of the canine herpesvirus thymidine kinase gene: taxon-preferred amino acid residues in the alphaherpesviral

The neonatal mortality rate in dogs is generally considered high although only a few studies have been published. Parts of the cranial mesenterial ganglion were included in the sections of the adrenal gland in seven cases. The 2 dogs were part of a group of 4 adult Golden Retrievers from the same household that were boarded together in a kennel. Treatment included antibiotics, fluids, antiemetics, antidepressants, and nonsteroidal anti-inflammatory drugs. The current study describes a case of fatal CaHV-1 infection in a 9-year-old spayed female Bichon Frise dog. The neonatal mortality rate in dogs is generally considered high although only a few studies have been published. Parts of the cranial mesenterial ganglion were included in the sections of the adrenal gland in seven cases.

Consequently, viral reactiva- tion in a seronegative dog cannot be excluded. Although the pathogenic potential of CHV for older dogs is apparently low and adult dogs often do not show any clinical signs following CHV infection, CHV may become an important perinatal pathogen for pregnant bitches, causing papulovesicular genital lesions and reproductive disorders such as embryonic resorption, abortion, and stillbirth (11). and Bicalho, R. Phone: 0044 (0) 1707-666 486. Clinical data indicate the circulation of CHV-1 among Brazilian dogs yet definitive diagnosis has rarely been accomplished. Electron microscopy revealed typical viral inclusions within bronchiolar epithelial cells. For CAdV, the age and seasonal patterns of seroprevalence were generally consistent with the recruitment of young susceptible foxes into the population in the spring and the accumulation of infections with age.


All pups were raised at the same facility with a common history but were from 2 separate litters. In addition, other agents such as CHV and CRCoV may play a role in the pathogenesis of canine respiratory disease, whereas CAV-2 and canine distemper virus were not present in this population, indicating that their prevalence in the United Kingdom is low due to widespread vaccination of dogs. Recently, work has focused on molecular characterisation of FHV-1, detecting genes such as glycoproteins or regulatory genes. In the alveolar spaces, large amounts of homogeneous eosinophilic material and numerous bacteria were observed in the absence of an associated inflammatory reaction. All Rights Reserved. Nasal secretions randomly collected during the cold season were tested via PCR, and this data collection was followed by nucleotide sequencing of the amplicons. Our major conclusion is that natural products from medicinal plant extracts are very important source of anti-HSV agents.

These results indicate the need for control/prevention measures, including vaccination and environmental management, to minimize these infections and improve dog health. Unlike other CHV-1-specific diagnostic methods, this quantitative assay permits simultaneous detection and quantitation of CHV-1 DNA in a wide range of canine tissues and body fluids, thus providing a useful tool for confirmation of a clinical diagnosis, for the study of viral pathogenesis and for evaluation of the efficacy of vaccines and antiviral drugs. The low prevalence of antibody to CaHV-1 suggests that CaHV-1 may be a more suitable vector than CAdV for bait delivery of immunocontraceptive antigens to foxes in Australia. The initial tracer uptake rate in C6tk cells was in the order FLT>FMAU>FEAU>FIAU>FHBG>FHPG, whereas the initial tracer uptake rate in C6 control cells was FLT>FMAU>FIAU>FEAU approximately = FHBG approximately = FHPG. Corneal microtrephination and subconjunctival corticosteroid administration did not significantly affect clinical disease or viral shedding. The same genomic organization was found as in MPV with the gene order 3′-NS1-NS2-N-P-M-SH-G-F-M2-L-5′ and with the coding regions being flanked by leader and trailer regions at the 3′ and 5′ ends (genome sense), respectively (Fig. Similar, ISH staining was mostly negative in these except for one case with a few positive cells.

Treatment is aimed primarily at palliative care, such as antitussive drugs, broad-spectrum antimicrobials (e.g. Our data demonstrate that ablation of CCSP(+) BMC delays airway recovery and suggests the beneficial effect of CCSP(+) BMC in lung recovery is in part due to production of CCSP itself. see Additional file 3 for details. Consequently, CHV has not generally been regarded to be a primary cause of canine ITB (9, 11). Competing interests: The authors have declared that no competing interests exist. In an assay designed to detect antibodies to EBV viral capsid antigens, 41 % of dogs were positive. Journal of Animal Science, 2016 12016Spontaneous reproductive tract lesions in aged captive chimpanzees BK Chaffee, AP Beck, MA Owston, S Kumar, WB Baze, ER Magden, …

To determine which CHV glycoprotein corresponds to gB, gC or gD, the putative genes of gB, gC, and gD of CHV were inserted into the thymidine kinase gene of vaccinia virus LC16mO strain under the control of the early-late promoter for the vaccinia virus 7.5-kilodalton polypeptide. The current case was observed in a litter of Jack Russell terrier dogs housed in a kennel in the Apulia region, Italy. Full-genomic Characterisation of the Italian CnPnV Prototype Strain The virus detected in one dog (dog/Bari/100-12/ITA/2012) was assumed as prototype of the CnPnV strains circulating in the shelter and submitted to full-genomic sequence analysis.