Seropositivity to human herpesvirus 8 in relation to sexual history and risk of sexually transmitted

The baseline characteristics and results are summarized in Table 3. The HHV-8 sero-status of 159 Tobago men, ages 40-65 years, were compared to that of the subset of Tobago women of comparable age (n = 122). Relationship of human immunodeficiency virus type 1 (HIV-1) and human herpesvirus 8 (HHV-8) serostatus in heterosexual Zimbabwean couples. This increase in KSHV genome copy number between days 7 and 14 after inoculation and subsequent reduction provides evidence for initial viral replication in the implant, as would be expected in a de novo infection. Indeed, the results of the present study support the association of HHV-8 infection with multiple homosexual contacts. Seroreactivity to LANA and ORF65 viral proteins seems to increase in elderly persons, but is almost equally distributed between men and women [7, 12]. We will include full text publications, but will also assess studies published only as abstracts.

In this study, the overall rate of HHV-8 seroprevalence in patients awaiting transplant was 14.8%. Despite rituximab therapy the patient died 4 months later from extensive KS associated with polyclonal HHV8-related lymphoproliferation. KSHV/HHV-8 encodes 12 microRNAs (miRNAs) coordinately expressed in the latency region. Stringent conditions were used to limit PCR contamination, as outlined in the “Conventional HHV-8, Ep-stein-Barr virus (EBV), and HIV-1 PCR” subsection above, and equivalently amplified BC-3 or BCBL-1 sequences were cloned and sequenced in parallel to serve as controls. 1 B shows a time course of infection. The risk associated with HHV-8 infection and markers of oro-fecal, blood-borne, or sexual transmission were estimated by use of seroprevalence ratios (SRs) and their 95% confidence intervals (CIs), adjusted for age group (≤14 years, 15–24 years, 25–34 years, and ≥35 years) and for sex. The prevalence of coinfection was 1.28% (95% CI, 0.03%–2.53%) among participants aged 17–21 years; prevalence was highest among participants aged 26–28 years (9.62%; 95% CI, 6.57%–12.7) and was similar to this level in the older age groups.


Variables with a P < 0.05 in univariable analysis were included in a multivariable logistic regression model utilizing a manual forward selection process, to control for possible confounders and identify independent associations. Phylogenetic analysis of a 647‐bp alignment corresponding to most of the available human herpesvirus 8 (HHV‐8) open‐reading frame (ORF) K1 fragment, including all subtype D and E HHV‐8 sequences. Primary neonatal and adult capillary endothelial cell cultures (kindly provided by Scott Heron) were also grown in Endothelial Cell Growth Medium (Clonetics Corporation). Coverslips were surrounded with mineral oil, and the slides were placed directly on the aluminum block of the thermocycler (Omnigene; Hybaid, Woodbridge, N.J.). Ndlovu, E.S.B. Phylogenetic analysis was performed with the neighbor-joining module of Geneious Pro. The lack of association found in this investigation between HHV-8 and HTLV-1 infections suggests that these viruses do not share similar transmission modalities. All individuals in the cohort were tested for herpes simplex types 1 and 2, hepatitis B, C and G by indirect ELISA (GBC ELISA provided by Dietmar Zdunek, Roche Diagnostics, Germany; HSV-2 ELISA Diasorin, Saluggia, Italy), syphilis (MHA-Tp and FTA-abs), CMV, EBV, toxoplasmosis (Diasorin, Saluggia, Italy). After 16 to 20 h the cells were pelleted and resuspended in the same amount of medium without TPA and ionomcycin. The peptides were first screened for IgG reactivity with serum pools from 5 different groups: lytic−latent−, lytic+latent−, lytic+latent+, healthy blood donors, and KS patients. (a) Penetrance (ie, probability of being HHV-8 seropositive) for children under the age of 15 years, as a function of age, genotype for the detected major gene (DD, two upper lines; Dd or dd, two lower lines) and HHV-8 status of preceding siblings. The nested PCR analysis was performed using 0.5 μL of the first-round product with the inner primer set. Nonetheless, the association of short-stored seropositive blood remained when the analysis was restricted to recipients of single transfusions or units or when number of transfusions was taken into account. Study population. Med. In comparison, 95.5% of the centers reported screening for EBV antibodies (ranging from 87.5% of centers in central Italy to 100% of centers in southern Italy). The current state of the literature is reviewed and knowledge gaps are identified for future exploration. Antibodies against either lytic antigen or LANA were detected in 79.1% of Amerindians and in 6.1% of non-Amerindians (adjusted seroprevalence ratio [SR], 12.63 [95% confidence interval {CI}, 7.1–22.4]; P< .0001). Little is known about the epidemiology of HHV8 in Europe before the HIV epidemic. Among the human immunodeficiency virus (HIV)-1-infected women, 51.1% were HHV-8-seropositive, whereas of HIV-1-negative women, 47.3% were HHV-8-seropositive. Epidemiologic, clinic, immunologic and virologic characteristics of 31 HIV infected patients with KS were included in this descriptively and retrospectively analysis from 2010 to 2013. After initial infection, gray cell (circled 1), virus spreads intercellularly (circled 2). Most virus particles ranged in size from 115 to 125nm. This article has been cited by other articles in PMC.